In Vitro Fertilization : A Textbook of Current and Emerging Methods and Devices
https://libcat.nshealth.ca/en/permalink/provcat44366
Zsolt Peter Nagy, Alex C. Varghese, Ashok Agarwal, editors.
(Second edition)
--Cham:
Springer
, c2019.
- Available Online
- View e-Book
- Location
- Online
Now in its revised and expanded second edition - including over 20 new chapters - this comprehensive textbook remains a unique and accessible description of the current and developing diagnostic and treatment techniques and technologies comprising in vitro fertilization (IVF). Arranged thematically in sections, each chapter covers a key topic in IVF in a sensible presentation. Parts one and two describe the planning, design and organization of an ART unit and IVF laboratory and equipment and sy…
- Available Online
- View e-Book
- Other Authors
- Nagy, Zsolt Peter
- Varghese, Alex C.
- Agarwal, Ashok
- Responsibility
- Zsolt Peter Nagy, Alex C. Varghese, Ashok Agarwal, editors
- Edition
- Second edition
- Place of Publication
- Cham
- Publisher
- Springer
- Date of Publication
- c2019
- Physical Description
- 1 online resource (xxii, 962 p.) : 213 illus., 198 illus. in color
- ISBN
- 9783319430119
- 9783319430102 (Print ed.)
- 9783319430126 (Print ed.)
- Subjects (MeSH)
- Clinical Laboratory Techniques
- Fertilization in Vitro - instrumentation
- Fertilization in Vitro - methods
- Specialty
- Reproductive Medicine
- Abstract
- Now in its revised and expanded second edition - including over 20 new chapters - this comprehensive textbook remains a unique and accessible description of the current and developing diagnostic and treatment techniques and technologies comprising in vitro fertilization (IVF). Arranged thematically in sections, each chapter covers a key topic in IVF in a sensible presentation. Parts one and two describe the planning, design and organization of an ART unit and IVF laboratory and equipment and systems, respectively. The sections that follow provide detailed descriptions of IVF techniques, embryo culture methods, sperm processing and selection, insemination procedures, micromanipulation, embryo evaluation, cryopreservation, and embryo transfer. Concluding sections address issues of management and regulation of ART labs across the globe, as well as special topics and emerging techniques and devices. Chapter authors, all experts in the field, contribute their expertise from around the world. With the addition of learning key points and review questions at the beginning and end of each chapter, this new edition of In Vitro Fertilization is a readily accessible, high quality instructional resource for reproductive medicine trainees at all levels. Practicing reproductive endocrinologists, urologists, and embryologists also will find value in the book, as will infertility researchers.
- Contents
- Journey of Human Gametes In Vitro: 1978 to 2018 -- I. Setting Up an ART Unit: Planning, Design and Organization -- Setting up an ART Unit - Planning, Design and Construction -- Building the Laboratory -- Air Quality Management -- Organization And Workflow In A Fertility Clinic -- Risk and Safety in the IVF Clinic -- Control of Variables -- Daily, Weekly, and Regular Preparations for the IVF Laboratory -- II. IVF Laboratory Equipment and Culture Systems -- Essential Instruments and Disposable Supplies for an IVF Laboratory -- Co2 and Low-O2 Incubators -- What to Consider When Selecting a LAF, Class II Cabinet or Isolette for Your ART Program? -- Culture Media in IVF: Decisions for the Laboratory -- III. Oocyte Preparation for IVF/ICSI -- Assessment Of Oocyte Quality -- Oocyte Denuding -- IV. Embryo Culture Methods -- Interrupted or Uninterrupted Culture to the Blastocyst Stage in a Single Medium -- Sequential Media for Human Blastocyst Culture -- In Vitro Maturation of Human Oocytes -- Design and Development of Simplified, Low Cost Technologies for Clinical IVF: Applications in High and Low Resource Settings -- In Vitro Culture of Oocytes and Embryos with Micro-vibration: “Naturalization” of Routine IVF Procedure -- Microfluidics for Gamete Manipulation and Embryo Culture -- Time-Lapse Microscopy for Embryo Culture and Selection -- V. Sperm Assessment, Processing and Selection -- Sperm Assessment: Traditional Approaches and Their Indicative Value -- Sperm Assessment: Novel Approaches and Their Indicative Value -- Assessment of Sperm Chromatin Damage by TUNEL Method Using Bench Top Flow Cytometer -- Sperm Processing in ART -- PESA/MESA/TESA/TESE Sperm Processing -- Processing Sperm Samples in HIV-Positive Patients -- An Emerging Medical Device: Electrophoretic Sperm Separation -- Magnetic Activated Cell Sorting of Human Spermatozoa -- VI. Insemination Procedures -- Intrauterine Insemination -- Conventional IVF Insemination -- Intra-Cytoplasmic Sperm Injection -- ntracytoplasmic Morphologically Selected Sperm Injection -- Intracytoplasmic Morphologically Selected Sperm Injection -- Oocyte Activation Deficiency and Advances to Overcome -- VII. Micromanipulators and Micromanipulation -- Hydraulic Manipulators for ICSI -- Research Instruments Micromanipulation Systems -- Eppendorf Micromanipulator: Setup and Operation of Electronic Micromanipulators.-Oocyte Treatment and Preparation for Microinjection -- Piezo-ICSI -- VIII. Embryo Evaluation, Grading, and Assisted Hatching -- Traditional Embryo Morphology Evaluation: From the Zygote to the Blastocyst Stage -- Predicting Embryo Implantation Potential Using Video Monitoring By the Embryoscope Time-Lapse System -- Predicting Embryo Developmental Potential and Viability Using Automated Time-Lapse Analysis -- Proteomics and Metabolomics -- Amino Acid Turnover as a Biomarker of Embryo Viability -- Microfluidics and Microanalytics to Facilitate Quantitative Assessment of Human Embryo Physiology -- Assisted Hatching of Human Embryos for Successful Implantation -- IX. Biopsy Procedures on Oocytes and Embryos -- Polar Body Biopsy -- Cleavage-Stage Embryo Biopsy -- Current and Novel Methods for Chromosome Testing -- Embryo Biopsy for PGD: Current Perspective -- X. Cryopreservation -- Sperm Cryopreservation -- Technologies of Cryoprotectant-free Vitrification of Human Spermatozoa: Asepticity as Criterionn of Effectiveness -- Slow Freezing of Oocytes -- Vitrification: Methods Contributing to Successful Cyopreservation Outcomes -- Oocyte Vitrification and Current Clinical Applications -- Slow Freezing of Embryos -- Vitrification of Embryos -- Ovarian Tissue Cryopreservation -- Establishing and Managing Donor Oocyte Banking -- XI. Embryo Transfer and Advanced Treatment Options -- Single Embryo Transfer -- Embryo Transfer: Techniques and Trouble shooting -- G-CSF and GM-CSF: Clinical Applications in Reproductive Medicine -- The Freeze All Cycle: A New Paradigm Shift in ART -- XII. Management and Regulation in the ART Laboratory -- IVF Data Management: From Clipboards to Smart Apps -- Regulation of the ART Laboratory -- Legislation in the UK -- Regulation, Licensing, and Accreditation of the ART Laboratory in Europe -- Regulation, Licensing, and Accreditation of the ART Laboratory in Brazil -- Regulation, Licensing and Accreditation of ART Laboratories in Australia -- Trouble Shooting In Ivf Laboratories -- Benchmarking and Decision Making in the IVF Laboratory -- XIII. Innovations, Risks and Safety in ART -- Automation, Artificial Intelligence and Innovations in the Future of IVF -- Assisted Reproductive Technologies to Prevent Transmission of Mitochondrial DNA Disease -- Nuclear and Cytoplasmic Transfer: Human Applications and Concerns -- Embryo Culture and Phenotype of the Offspring -- Traceability in ART -- XIV. Molecular Reproduction -- The Role of Mitochondria in the Establishment of Developmental Competence in Early Human Development -- Cytoskeletal Architecture of Human Oocytes with a Focus on Centrosomes and Their Significant Role in Fertilization -- Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence -- Livestock Production via Micromanipulation.
- Format
- e-Book
- Location
- Online
Minimal Stimulation and Natural Cycle In Vitro Fertilization
https://libcat.nshealth.ca/en/permalink/provcat39498
Gautam N. Allahbadia, Markus Nitzschke, editors.
(1st ed.)
--New Delhi:
Springer India
, c2015.
- Available Online
- View e-Book
- Location
- Online
This contributed volume regenerates the significance of and respect for natural phenomenon in the background of the risks and complications seen in ART such as ovarian hyperstimulation and multiple pregnancies. Balancing the clinical practice between the beneficial natural physiology and the prolific use of stimulation drugs for ART may help eliminate risks that may prove clinically, financially and psychologically expensive. It underscores the fact that "greater" is not always synonymous with …
- Available Online
- View e-Book
- Other Authors
- Allahbadia, Gautam N
- Nitzschke, Markus
- Responsibility
- Gautam N. Allahbadia, Markus Nitzschke, editors
- Edition
- 1st ed.
- Place of Publication
- New Delhi
- Publisher
- Springer India
- Date of Publication
- c2015
- Physical Description
- 1 online resource (xvi, 101 p. : 17 illus., 9 illus. in color)
- ISBN
- 9788132211181
- 9788132211174 (print ed.)
- Subjects (MeSH)
- Fertilization in Vitro - methods
- Infertility - therapy
- Ovulation Induction - methods
- Abstract
- This contributed volume regenerates the significance of and respect for natural phenomenon in the background of the risks and complications seen in ART such as ovarian hyperstimulation and multiple pregnancies. Balancing the clinical practice between the beneficial natural physiology and the prolific use of stimulation drugs for ART may help eliminate risks that may prove clinically, financially and psychologically expensive. It underscores the fact that "greater" is not always synonymous with "better" with regard to the oocyte yield. While highlighting the risks involved with the use of high-dose gonadotropins, the book presents a stratification of patients who might benefit from the "soft" minimal and natural cycle IVF stimulation protocols that may be used. Additionally, clinicians can understand the significance of cryopreservation technology, now a useful adjunct to minimal stimulation protocols, with its manifold future advantages. Written by an international team of experts, this handbook emphasizes how minimal stimulation and natural cycle IVF can lead to a milder, safer and more effective approach to ART.
- Contents
- 1. ART Stimulation in the Next Decade: An Overview -- 2. Natural Cycle IVF: An Overview -- 3. Natural Cycle IVF: Follicle Physiology and Treatment Costs -- 4. The Use of Clomiphene Citrate in Natural Cycle IVF -- 5. The Role of Non-steroidal Anti- inflammatory Drugs in Natural Cycle IVF -- 6. Theoretical Backgrounds of the Natural Cycle and Other Minimal Stimulation Cycles: From Follicle Observation to Embryo Transfer -- 7. Ovarian Stimulation in Patients with Ovarian Insufficiency -- 8. Monofollicular Stimulation in PCOS Patients -- 9. Luteal Phase Support in Natural Cycle and Minimal Stimulation IVF -- 10. Budget IVF Using Clomiphene, hMG, and Antagonists -- 11. Vitrification Is the Keystone to Minimal Stimulation IVF -- 12. Lessons Learned and Future Challenges.
- Format
- e-Book
- Location
- Online
Practical Manual of In Vitro Fertilization : Advanced Methods and Novel Devices
https://libcat.nshealth.ca/en/permalink/provcat30849
Zsolt Peter Nagy, Alex C. Varghese, Ashok Agarwal, editors.
--New York, NY:
Springer
, c2012.
- Available Online
- View e-Book
- Location
- Online
The Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices is a unique, accessible title that provides a complete review of the most well-established and current diagnostic and treatment techniques comprising in vitro fertilization. Throughout the chapters, a uniform structure is employed, including a brief abstract, a keyword glossary, a step-by-step protocol of the laboratory procedures, several pages of expert commentary, key issues of clinical concern, and a list of …
- Available Online
- View e-Book
- Other Authors
- Nagy, Zsolt Peter
- Varghese, Alex C
- Agarwal, Ashok
- Responsibility
- Zsolt Peter Nagy, Alex C. Varghese, Ashok Agarwal, editors
- Place of Publication
- New York, NY
- Publisher
- Springer
- Date of Publication
- c2012
- Physical Description
- 1 online resource (xxiii, 703 p. : 139 ill., 103 ill. in color)
- ISBN
- 9781441917805
- Subjects (MeSH)
- Clinical Laboratory Techniques
- Fertilization in Vitro - methods
- Subjects (LCSH)
- Obstetrics
- Cytology
- Endocrinology
- Developmental biology
- Urology
- Abstract
- The Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices is a unique, accessible title that provides a complete review of the most well-established and current diagnostic and treatment techniques comprising in vitro fertilization. Throughout the chapters, a uniform structure is employed, including a brief abstract, a keyword glossary, a step-by-step protocol of the laboratory procedures, several pages of expert commentary, key issues of clinical concern, and a list of references. The result is a readily accessible, high quality reference guide for reproductive endocrinologists, urologists, embryologists, biologists and research scientists. The Manual also offers an excellent description of novel procedures that will likely be employed in the near future. An indispensable resource for physicians and basic scientists, the Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices is an invaluable reference and addition to the literature.
- Contents
- Journey of Human Gametes In Vitro: 1978 to 2010 -- Part I. Setting Up and Running an IVF Laboratory -- Building the Laboratory -- Air Quality Management -- Organizational Aspects of the Laboratory in a Tertiary Care ART Center -- Quality Control Management -- Daily, Weekly, and Regular Preparations for the IVF Laboratory -- Risk and Safety in the IVF Clinic -- Part II. IVF Laboratory Equipment and Culture Systems -- Essential Instruments and Disposable Supplies for an IVF Laboratory -- Co2 and Low-O2 Incubators -- IVF Workstations -- Culture Media in IVF: Decisions for the Laboratory -- Part III. In Vitro Fertilization -- Oocyte Denuding -- Assessment of Oocyte Quality -- Polarization Microscopy -- Cumulus Cell Gene Expression in Assessment of Oocyte Quality -- Part IV. Embryo Culture Methods -- Short Culture: Day 1/Day 2/Day 3 Embryo Culture -- Extended Culture in IVF -- In Vitro Maturation of Human Oocytes -- In Vivo Embryo Culture Device -- Microfluidics for Gamete Manipulation and Embryo Culture -- Part V. Sperm Processing and Selection -- Sperm Assessment: Traditional Approaches and Their Indicative Value -- Sperm Assessment: Novel Approaches and Their Indicative Value -- Sperm Processing for IVF -- PESA/TESA/TESE Sperm Processing -- Processing Sperm Samples in HIV-Positive Patients -- Intracytoplasmic Morphologically Selected Sperm Injection -- Sperm Testing and ICSI Selection by Hyaluronic Acid Binding: The Hyaluronic Acid-Coated Glass Slide and Petri Dish in the Andrology and IVF Laboratories -- Electrophoretic Sperm Separation -- Magnetic Activated Cell Sorting of Human Spermatozoa -- Poloscope-Based Sperm Selection -- Part VI. Insemination Procedures -- Intrauterine Insemination -- Conventional IVF Insemination -- Intracytoplasmic Sperm Injection -- Mechanism of Human Oocyte Activation During ICSI and Methodology for Overcoming Low or Failed Fertilization -- Part VII. Micromanipulators and Micromanipulation -- Hydraulic Manipulators for ICSI -- Research Instruments Micromanipulators -- Eppendorf Micromanipulator: Setup and Operation of Electronic Micromanipulators -- The Leica-IMSI System -- Automated Robotic Intracytoplasmic Sperm Injection -- Oocyte Treatment and Preparation for Microinjection -- Livestock Production Via Micromanipulation -- Part VIII. Embryo Evaluation, Grading, and Assisted Hatching -- Pronuclear Scoring in Human In Vitro Fertilization -- Cumulative Morphological Assessment of Embryo Quality -- Metabolomics: The ViaMetrics-E™ Procedure for Assessing Embryo Viability -- Oxygen Consumption as an Indicator of Oocyte and Embryo Viability -- Gene Expression Changes During Human Early Embryo Development: New Applications for Embryo Selection -- Amino Acid Turnover as a Biomarker of Embryo Viability -- Real-time Embryo Monitoring Devices for Embryo Selection -- Assisted Hatching in IVF -- Part IX. Biopsy Procedures on Oocytes and Embryos -- Polar Body Biopsy -- Cleavage Stage Embryo Biopsy -- Embryo Biopsy for PGD: Current Perspective -- Microarrays and CGH for PGD of Chromosome Abnormalities and Gene Defects -- Part X. Cryopreservation -- Sperm Cryopreservation -- Slow Freezing of Oocytes -- Vitrification: Research in Animal Models -- Oocyte Vitrification -- Slow Freezing of Embryos -- Vitrification of Embryos -- Directional Freezing of Reproduction Cells, Tissues, and Organs -- Ovarian Tissue Cryopreservation -- Part XI. Embryo Transfer -- Assessment of Uterine Receptivity -- Single Embryo Transfer -- Ultrasound-Guided Embryo Transfer (Abdominal/Vaginal): An Evidence-Based Evaluation -- Cumulus-Aided Embryo Transfer -- Part XII. Management and Regulation in the ART Laboratory -- Data Management in the ART Laboratory: Requirements and Solutions -- Regulation, Licensing, and Accreditation of the ART Laboratory -- Legislation in the United Kingdom -- Regulation, Licensing, and Accreditation of the ART Laboratory in Europe -- Regulation, Licensing, and Accreditation of ART Laboratories in India -- Troubleshooting in the Clinical Embryology Laboratory: The Art of Problem-Solving in ART -- Part XIII. Special Topics -- The Role of Mitochondria in the Establishment of Developmental Competence in Early Human Development -- Nuclear and Cytoplasmic Transfer: Human Applications and Concerns -- Cytoskeletal Architecture of Human Oocytes with Focus on Centrosomes and Their Significant Role in Fertilization -- Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence.
- Format
- e-Book
- Location
- Online
