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Atlas of Confocal Laser Scanning In-vivo Microscopy in Ophthalmology : Principles and Applications in Diagnostic and Therapeutic Ophthalmology

https://libcat.nshealth.ca/en/permalink/provcat29266
by Rudolf F. Guthoff, Christophe Baudouin, Joachim Stave. --Berlin, Heidelberg: Springer-Verlag , c2006.
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Location
Online
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Author
Guthoff, Rudolf F
Other Authors
Baudouin, Christophe
Stave, Joachim
Responsibility
by Rudolf F. Guthoff, Christophe Baudouin, Joachim Stave
Place of Publication
Berlin, Heidelberg
Publisher
Springer-Verlag
Date of Publication
c2006
ISBN
9783540327073
Subjects (MeSH)
Microscopy, Confocal - methods
Eye Diseases - diagnosis
Conjunctiva - cytology
Conjunctiva - pathology
Cornea - cytology
Cornea - pathology
Subjects (LCSH)
Ophthalmology
Format
e-Book
Location
Online
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Atlas of Endomicroscopy

https://libcat.nshealth.ca/en/permalink/provcat28071
by Ralf Kiesslich, Peter R. Galle, Markus Neurath. --Berlin, Heidelberg: Springer Medizin Verlag , c2008.
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Location
Online
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Author
Kiesslich, Ralf
Other Authors
Galle, Peter R
Neurath, Markus
Responsibility
by Ralf Kiesslich, Peter R. Galle, Markus Neurath
Place of Publication
Berlin, Heidelberg
Publisher
Springer Medizin Verlag
Date of Publication
c2008
ISBN
9783540351153
Subjects (MeSH)
Gastroscopy - atlases
Endoscopy, Gastrointestinal - Atlases
Microscopy - methods - Atlases
Subjects (LCSH)
Gastroenterology
Endoscopic surgery
Format
e-Book
Location
Online
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Atlas of Human Semen Examination

https://libcat.nshealth.ca/en/permalink/provcat45615
Donatella Paoli, Francesco Lombardo, Andrea Lenzi. --Cham: Springer , c2020.
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This atlas provides valuable information on crucial aspects of sperm examination as well numerous meaningful color illustrations. It discusses successful evaluation of the sperm morphology and the cellular elements other than spermatozoa, enabling readers to unambiguously interpret seminal cytologic images and compare reports for diagnostic, therapeutic and prognostic purposes. With its extensive collection of colored images, the book is intended as a reference resource for students and technic…
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Author
Paoli, Donatella
Other Authors
Lombardo, Francesco
Lenzi, Andrea
Responsibility
Donatella Paoli, Francesco Lombardo, Andrea Lenzi
Place of Publication
Cham
Publisher
Springer
Date of Publication
c2020
Physical Description
1 online resource (vii, 106 p.) : 172 illus. in color
Series Title
Trends in andrology and sexual medicine
ISBN
9783030399986
9783030399979 (Print ed.)
9783030399993 (Print ed.)
9783030400002 (Print ed.)
ISSN
2367-0088
Subjects (MeSH)
Cytodiagnosis
Cytological Techniques
Microscopy - methods
Spermatozoa - ultrastructure
Specialty
Men's Health
Microscopy
Reproductive Medicine
Abstract
This atlas provides valuable information on crucial aspects of sperm examination as well numerous meaningful color illustrations. It discusses successful evaluation of the sperm morphology and the cellular elements other than spermatozoa, enabling readers to unambiguously interpret seminal cytologic images and compare reports for diagnostic, therapeutic and prognostic purposes. With its extensive collection of colored images, the book is intended as a reference resource for students and technicians in the field of andrology as well as practitioners and clinicians in andrology, urology, pathology, IVF and other ART programs.
Contents
Introduction -- Human Semen Examination -- 1. Notes on Human Spermatogenesis -- 2. Morphological Structure of the Spermatozoon -- 3. Non-sperm Cellular Components of the Seminal Fluid -- 3.1 Elements from the Spermatogenesis Process -- 4. Morphological Study of the Seminal Fluid -- 4.1 Preparation of Semen Smears -- 4.2 Staining Techniques -- 5. Chromatin Aspect -- 5.1 Chromatin Organization in Human Sperm -- Further Reading -- Image Gallery: Sperm Morphology -- Image Gallery: Non-Sperm Cellular Components -- Image Gallery: Chromatin Integrity -- Image Gallery: Morphological Evaluation of Sperm Heads with Fragmented DNA, Using Both Transmitted (Bright Field) and Reflected (Dark Field) Light; Every Picture is Shown in Three Different Light Condition.
Format
e-Book
Publication Type
Atlas
Location
Online
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Bioimage Data Analysis Workflows

https://libcat.nshealth.ca/en/permalink/provcat44717
Kota Miura, Nataša Sladoje, editors. --Cham: Springer , c2020.
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Open access
Location
Online
This Open Access textbook provides students and researchers in the life sciences with essential practical information on how to quantitatively analyze data images. It refrains from focusing on theory, and instead uses practical examples and step-by step protocols to familiarize readers with the most commonly used image processing and analysis platforms such as ImageJ, MatLab and Python. Besides gaining knowhow on algorithm usage, readers will learn how to create an analysis pipeline by scriptin…
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Other Authors
Miura, Kota
Sladoje, Nataša
Responsibility
Kota Miura, Nataša Sladoje, editors
Place of Publication
Cham
Publisher
Springer
Date of Publication
c2020
Physical Description
1 online resource (x, 170 p.) : 69 illus., 58 illus. in color
Series Title
Learning materials in biosciences
ISBN
9783030223861
9783030223854 (Print ed.)
9783030223878 (Print ed.)
ISSN
2509-6125
Subjects (MeSH)
Biomedical Technology
Image Interpretation, Computer-Assisted - methods
Medical Informatics
Microscopy
Workflow
Subjects (LCSH)
Biomedical engineering
Cell biology
Bioinformatics
Biology—Technique
Systems biology
Biological systems
Specialty
Diagnostic Imaging
Medical Informatics
Abstract
This Open Access textbook provides students and researchers in the life sciences with essential practical information on how to quantitatively analyze data images. It refrains from focusing on theory, and instead uses practical examples and step-by step protocols to familiarize readers with the most commonly used image processing and analysis platforms such as ImageJ, MatLab and Python. Besides gaining knowhow on algorithm usage, readers will learn how to create an analysis pipeline by scripting language. These skills are important in order to document reproducible image analysis workflows. The textbook is chiefly intended for advanced undergraduates in the life sciences and biomedicine without a theoretical background in data analysis, as well as for postdocs, staff scientists and faculty members who need to perform regular quantitative analyses of microscopy images.
Contents
Workflows and Components of Bioimage Analysis -- Measurements of Intensity Dynamics at the Periphery of the Nucleus -- 3D Quantitative Colocalisation Analysis -- The NEMO Dots Assembly: Single-Particle Tracking and Analysis -- Introduction to MATLAB -- Resolving the Process of Clathrin Mediated Endocytosis Using Correlative Light & Electron Microscopy (CLEM).
Access
Open access
Format
e-Book
Location
Online
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Cochlear Anatomy via Microdissection with Clinical Implications : An Atlas

https://libcat.nshealth.ca/en/permalink/provcat42999
Charles G. Wright, Peter S. Roland. --Cham: Springer , c2018.
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This atlas focuses on selected aspects of cochlear anatomy as illustrated by material prepared by the microdissection technique, a three-dimensional perspective not possible with standard histological approaches. Although much of the material in this text was processed by the microdissection method, photomicrographs from conventionally cross sectioned inner ear tissues and scanning electron microscopy, are also presented. Taken together, these technical approaches provide different, complementa…
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Author
Wright, Charles G
Other Authors
Roland, Peter S
Responsibility
Charles G. Wright, Peter S. Roland
Place of Publication
Cham
Publisher
Springer
Date of Publication
c2018
Physical Description
1 online resource (ix, 115 p.) : 122 illus., 103 illus. in color
ISBN
9783319712222
9783319712215 (print ed.)
9783319712239 (print ed.)
Subjects (MeSH)
Cochlea - anatomy & histology
Cochlea - surgery
Microdissection - methods
Microscopy, Electron, Scanning - methods
Specialty
Anatomy
Otolaryngology
Abstract
This atlas focuses on selected aspects of cochlear anatomy as illustrated by material prepared by the microdissection technique, a three-dimensional perspective not possible with standard histological approaches. Although much of the material in this text was processed by the microdissection method, photomicrographs from conventionally cross sectioned inner ear tissues and scanning electron microscopy, are also presented. Taken together, these technical approaches provide different, complementary views of inner ear anatomy, and offer a more informative understanding than is possible with any of the methods used alone. While micrographs obtained from microdissected material appear sporadically in journal articles, there is no comprehensive collection of such images currently available. The illustrations assembled in this atlas are complementary to the more traditional histologic images and drawings available in standard texts, and aide in understanding the intricate anatomy of the cochlea. Cochlear Anatomy via Microdissection with Clinical Implications will be a useful resource for otolaryngologists, anatomists, audiologists, and neuroscientists. Those engaged in cochlear implantation, physicians and researchers in the growing fields of implantable hearing aids and drug delivery to the inner ear, and members of industry involved in designing, manufacturing, and marketing implantable hearing aids will also find this atlas of great value.
Contents
1. Microdissection for Study of Cochlear Anatomy -- 2. Anatomy of the Helicotrema and Cochlear Apex -- 3. Microdissection in Cochlear Implant Research -- 4. Implantation via the Round Window and Cochlear Hook Anatomy -- 5. Scala Tympani Vasculature and Possible Vascular Injury During Cochlear Implantation.
Format
e-Book
Publication Type
Atlas
Location
Online
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Color atlas of cytology, histology, and microscopic anatomy

https://libcat.nshealth.ca/en/permalink/provcat27557
Wolfgang Kuehnel ; [translated by Ursula Peter-Czichi]. (4th ed., rev. and enlarged) --Stuttgart: Thieme , c2003.
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Location
Online
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Author
Kuehnel, Wolfgang
Responsibility
Wolfgang Kuehnel ; [translated by Ursula Peter-Czichi]
Edition
4th ed., rev. and enlarged
Alternate Title
Colour atlas of cytology, histology, and microscopic anatomy
Cytology, histology, and microscopic anatomy
Place of Publication
Stuttgart
Publisher
Thieme
Date of Publication
c2003
Physical Description
1 online resource (ix, 534 p.)
ISBN
9781588901750
9781604061253 (eISBN)
Subjects (MeSH)
Cytology - Atlases
Histology - Atlases
Cells - ultrastructure - Atlases
Specialty
Cell Biology
Histology
Microscopy
Format
e-Book
Location
Online
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Cytopreparation : principles & practice

https://libcat.nshealth.ca/en/permalink/provcat32409
Gary W. Gill, author. --New York, NY: Springer , c2013.
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Cytopreparation: Principles & Practice by Gary W. Gill fills a long-standing need for an easy-to-use and authoritative manual on the fundamentals of cytopreparation up-to-and- including microscopy, screening, and data analysis. The text describes in phenomenological terms the most common materials and methods of specimen collection through mounting for gyn, non-gyn, and FNA specimens, as well as the underlying mechanistic bases. The author provides his expertise and information that will empowe…
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Author
Gill, Gary W
Responsibility
Gary W. Gill, author
Place of Publication
New York, NY
Publisher
Springer
Date of Publication
c2013
Physical Description
1 online resource (xxx, 437 p. : 131 ill., 106 ill. in color)
Series
Essentials in cytopathology
Series Vol.
v. 12
Series Title
Essentials in cytopathology series
ISBN
9781461449331
Subjects (MeSH)
Cytological Techniques - methods
Subjects (LCSH)
Medical laboratories
Pathology
Microscopy
Abstract
Cytopreparation: Principles & Practice by Gary W. Gill fills a long-standing need for an easy-to-use and authoritative manual on the fundamentals of cytopreparation up-to-and- including microscopy, screening, and data analysis. The text describes in phenomenological terms the most common materials and methods of specimen collection through mounting for gyn, non-gyn, and FNA specimens, as well as the underlying mechanistic bases. The author provides his expertise and information that will empower and enable readers to review and improve their laboratories' cytopreparatory techniques as they apply to the vast majority of specimens. This unique volume provides facts that are not readily available anywhere. Cytopreparation: Principles & Practice is intended for everyone associated with, and involved in, making cytologic preparations that are useful for their intended purpose. It will serve as a valuable reference tool for educators in cytology and histology, cytotechnology and histotechnology students, cytotechnologists, cytopreparatory technicians, cytopathologists, anatomical/clinical pathologists, pathology residents and cytopathology fellows.
Contents
Introduction -- Quality Control and Quality Assessment -- Specimen Collection -- Salt Solutions -- Slide Preparation -- Cytocentrifugation -- Membrane Filtration -- Fixation -- Cell Block Preparation -- Papanicolaou Stain -- Cross-contamination Control -- H&E Stain -- Romanowsky Stains -- Special Stains -- Clearing -- Mounting Media -- Cover Glasses -- Mounting -- Kœhler Illumination -- Screening -- Bethesda System 2001, CLIA '88, and Data Analysis -- APPENDIX -- Index.
Format
e-Book
Location
Online
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Dermoscopy : the essentials

https://libcat.nshealth.ca/en/permalink/provcat31806
Peter Soyer, Guiseppe Argenziano, Rainer Hofmann-Wellenhof ... [et al.]. (3rd ed.) --Philadelphia, PA: Elsevier , c2020.
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Registration required for PDF access.
Location
Online
Dermoscopy: The Essentials presents the practical guidance you need to master this highly effective, more economical, and less invasive alternative to biopsy. Drs. Peter Soyer, Giuseppe Argenziano, Rainer Hofmann-Wellenhof, and Iris Zalaudek explain all aspects of performing dermoscopy and interpreting results. With approximately 30% new clinical and dermoscopic images, valuable pearls and checklists, and online access to the fully searchable and downloadable text, you’ll have everything you n…
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Other Authors
Soyer, H. Peter
Responsibility
Peter Soyer, Guiseppe Argenziano, Rainer Hofmann-Wellenhof ... [et al.]
Edition
3rd ed.
Place of Publication
Philadelphia, PA
Publisher
Elsevier
Date of Publication
c2020
Physical Description
1 online resource, 352 p.
ISBN
9780702068829
Subjects (MeSH)
Skin Neoplasms - diagnosis
Melanoma - diagnosis
Microscopy - methods
Subjects (LCSH)
Skin - Cancer - Diagnosis
Skin Neoplasms - diagnosis
Melanoma - diagnosis
Microscopy - methods
Specialty
Dermatology
Diagnosis
Microscopy
Abstract
Dermoscopy: The Essentials presents the practical guidance you need to master this highly effective, more economical, and less invasive alternative to biopsy. Drs. Peter Soyer, Giuseppe Argenziano, Rainer Hofmann-Wellenhof, and Iris Zalaudek explain all aspects of performing dermoscopy and interpreting results. With approximately 30% new clinical and dermoscopic images, valuable pearls and checklists, and online access to the fully searchable and downloadable text, you’ll have everything you need to diagnose earlier and more accurately.
Access
Registration required for PDF access.
Format
e-Book
Location
Online
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Dermoscopy : the essentials

https://libcat.nshealth.ca/en/permalink/provcat50599
Soyer, Peter. (3rd ed.) --Philadelphia, PA: Elsevier , 2020.
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Online
Available Online
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Author
Soyer, Peter
Edition
3rd ed.
Place of Publication
Philadelphia, PA
Publisher
Elsevier
Date of Publication
2020
ISBN
9780702068829
Subjects (MeSH)
Skin Neoplasms - diagnosis
Melanoma - diagnosis
Microscopy - methods
Notes
Access to this online resource is restricted to IWK Health Centre employees working from inside the hospital network.
Format
e-Book
Location
Online
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Duvernoy's Atlas of the Human Brain Stem and Cerebellum : High-Field MRI : Surface Anatomy, Internal Structure, Vascularization and 3D Sectional Anatomy

https://libcat.nshealth.ca/en/permalink/provcat29025
by Thomas P. Naidich, Henri M. Duvernoy, Bradley N. Delman, A. Gregory Sorensen, Spyros S. Kollias, E. Mark Haacke. --Vienna: Springer , c2009.
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Location
Online
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Author
Naidich, Thomas P
Other Authors
Duvernoy, Henri M
Delman, Bradley N
Sorensen, A. Gregory
Kollias, Spyros S
Haacke, E. Mark
Responsibility
by Thomas P. Naidich, Henri M. Duvernoy, Bradley N. Delman, A. Gregory Sorensen, Spyros S. Kollias, E. Mark Haacke
Place of Publication
Vienna
Publisher
Springer
Date of Publication
c2009
ISBN
9783211739716
Subjects (MeSH)
Brain Stem - anatomy & histology - Atlases
Brain stem - physiology
Cerebellum - anatomy & histology - Atlases
Cerebellum - physiology
Magnetic Resonance Imaging - methods - Atlases
Microscopy - methods - Atlases
Subjects (LCSH)
Neurosciences
Format
e-Book
Location
Online
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Eyelid and Conjunctival Tumors : In Vivo Confocal Microscopy

https://libcat.nshealth.ca/en/permalink/provcat45409
Mathilde Kaspi, Elisa Cinotti, Jean-Luc Perrot, Thibaud Garcin, editors. --Cham: Springer , c2020.
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Online
This atlas gives the complete expert opinion on the diagnostic features of eyelid and conjunctival tumors (benign and malignant): a state-of-the-art guide with numerous images, useful for both dermatologists and ophthalmologists. This invaluable resource, illustrating clinical, histological and re fectance confocal microscopy features, first addresses the normal conditions of the ocular surface, then reviews lesions due to epidermal, melanocytic and adnexal tumors. A final part is devoted to co…
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Other Authors
Kaspi, Mathilde
Cinotti, Elisa
Perrot, Jean-Luc
Garcin, Thibaud
Responsibility
Mathilde Kaspi, Elisa Cinotti, Jean-Luc Perrot, Thibaud Garcin, editors
Alternate Title
Eyelid and conjunctival tumours
Place of Publication
Cham
Publisher
Springer
Date of Publication
c2020
Physical Description
1 online resource (viii, 134 p.) : 132 illus. in color
ISBN
9783030366063
9783030366056 (Print ed.)
9783030366070 (Print ed.)
9783030366087 (Print ed.)
Subjects (MeSH)
Conjunctival Neoplasms - diagnosis
Eyelid Neoplasms - diagnosis
Microscopy, Confocal
Specialty
Medical Oncology
Ophthalmology
Abstract
This atlas gives the complete expert opinion on the diagnostic features of eyelid and conjunctival tumors (benign and malignant): a state-of-the-art guide with numerous images, useful for both dermatologists and ophthalmologists. This invaluable resource, illustrating clinical, histological and re fectance confocal microscopy features, first addresses the normal conditions of the ocular surface, then reviews lesions due to epidermal, melanocytic and adnexal tumors. A final part is devoted to conjunctiva conditions, from normal to malignant conjunctival tumors. The high number of illustrations and their description of many ocular surface lesions with in vivo confocal microscopy make this atlas an essential guide for the practitioners of both specialities.
Contents
Part I. Prerequisites to Confocal Microscopy for the Human Eye -- 1. An Introduction to In Vivo Confocal Microscopy -- 2. The Normal Eyelid -- 3. The Normal Conjunctiva -- Part II. Epidermal Lesions of the Eyelid -- 4. Benign Epidermal Lesions -- 5. Precancerous Epidermal Lesions -- 6. Malignant Epidermal Tumors -- Part III. Melanocytic Lesions of the Eyelid -- 7. Benign Lesions with Basal Melanocyte Proliferation -- 8. Benign Melanocytic Tumors -- 9. Malignant Melanocytic Tumors -- Part I.V Non-epidermal and Non-melanocytic Lesions of the Eyelid -- 10. Appendageal Tumors -- 11. Vascular Tumors -- 12. Miscellaneous Tumors -- Part V. Conjunctival Tumors -- 13. Epithelial Conjunctival Tumors -- 14. Melanocytic Conjunctival Tumors -- 15. Non-epithelial and Non-melanocytic Conjunctival Tumors.
Format
e-Book
Publication Type
Atlas
Location
Online
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Fluorescence Lifetime Imaging Ophthalmoscopy

https://libcat.nshealth.ca/en/permalink/provcat44672
Martin Zinkernagel, Chantal Dysli, editors. --Cham: Springer , c2019.
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Location
Online
This book focuses on the emerging non-invasive imaging technique of Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO). FLIO reveals unique information on retinal diseases, ranging from age-related macular degeneration and vascular diseases to hereditary retinal dystrophies. Fluorescence lifetimes enable the evaluation of disease progression before irreversible structural changes occur. The image acquisition is suitable for diagnostic purposes and follow-up examinations to investigate the natu…
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Other Authors
Zinkernagel, Martin
Dysli, Chantal
Responsibility
Martin Zinkernagel, Chantal Dysli, editors
Place of Publication
Cham
Publisher
Springer
Date of Publication
c2019
Physical Description
1 online resource (xii, 121 p.) : 33 illus., 31 illus. in color
ISBN
9783030228781
9783030228774 (Print ed.)
9783030228798 (Print ed.)
9783030228804 (Print ed.)
Subjects (MeSH)
Disease Progression
Fluorescein Angiography
Microscopy, Confocal
Ophthalmoscopy
Retinal Diseases - diagnostic imaging
Specialty
Diagnostic Imaging
Ophthalmology
Abstract
This book focuses on the emerging non-invasive imaging technique of Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO). FLIO reveals unique information on retinal diseases, ranging from age-related macular degeneration and vascular diseases to hereditary retinal dystrophies. Fluorescence lifetimes enable the evaluation of disease progression before irreversible structural changes occur. The image acquisition is suitable for diagnostic purposes and follow-up examinations to investigate the natural course of disease, and to monitor the effects of possible therapies. This book fills the gap between available literature and gives state-of-the-art guidance on the principles of the FLIO technique, image acquisition, and data analysis. Written by a team of expert leaders within this field, this book will be relevant for scientists and clinicians with an interest in ophthalmoscopy.
Contents
1. Introduction -- 2. FLIO Technique and Principles -- 3. FLIO Historical Background -- 4. Fluorescence Lifetime Imaging Microscopy -- 5. The Fluorescence Lifetime Imaging Ophthalmoscope -- 6. Image Acquisition and Analysis -- 7. Fluorophores in the Eye -- 8. FLIO in the Healthy Eye -- 9. FLIO in Retinal Diseases -- 10. Age-Related Macular Degeneration - 11. FLIO in Diabetic Retinopathy - 12. Retinal Artery Occlusion -- 13. Central Serous Chorioretinopathy -- 14. Macular Telangiectasia Type 2 -- 15. Hereditary Retinal Diseases: Stargardt, Choroideremia, Retinitis Pigmentosa -- 16. Macular Pigment -- 17. Fluorescence Lifetime Imaging Ophthalmoscopy in Alzheimer’s Disease -- 18. FLIO in Mouse Models -- 19. Discussion and Summary.
Format
e-Book
Location
Online
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In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs

https://libcat.nshealth.ca/en/permalink/provcat40408
Shinichi Ohno, Nobuhiko Ohno, Nobuo Terada, editors. (1st ed.) --Tokyo: Springer Japan , c2016.
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Location
Online
This book focuses on actual morphofunctional findings of cells and tissues in living animal organs. Medical and biological scientists need to know the real in vivo morphology and immunolocalization of the molecular components in living animal organs. Recently, the live imaging of cells and tissues of animals with fluorescence-labeled proteins by gene manipulation has become more and more popular in biological fields. Current research, meanwhile, has revealed that immunohistochemical or morpholo…
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Other Authors
Ohno, Shinichi
Ohno, Nobuhiko
Terada, Nobuo
Responsibility
Shinichi Ohno, Nobuhiko Ohno, Nobuo Terada, editors
Edition
1st ed.
Place of Publication
Tokyo
Publisher
Springer Japan
Date of Publication
c2016
Physical Description
1 online resource (xi, 300 p. : 114 illus., 99 illus. in color)
ISBN
9784431557234
9784431557227 (print ed.)
Subjects (MeSH)
Animal Structures - anatomy & histology
Biopsy - methods
Cryoelectron Microscopy - methods
Freeze Substitution - methods
Intravital Microscopy - methods
Mice - anatomy & histology
Abstract
This book focuses on actual morphofunctional findings of cells and tissues in living animal organs. Medical and biological scientists need to know the real in vivo morphology and immunolocalization of the molecular components in living animal organs. Recently, the live imaging of cells and tissues of animals with fluorescence-labeled proteins by gene manipulation has become more and more popular in biological fields. Current research, meanwhile, has revealed that immunohistochemical or morphological studies exclusively depend on living animal organs. The cryotechnique is one of the most useful tools for immunohistochemistry and bioimaging of animal organs. This book describes the epoch-making cryotechnique originally developed by the editors. The book also makes the management of living animal morphology more accessible not only for biomedical researchers but also for clinical doctors, providing a valuable resource work on the current perspectives of in vivo morphology.
Contents
Part I. Overview -- 1. Introduction -- 2. Biomedical significance and development of "IVCT" -- 3. How to perform "IVCT" -- 4. Technical merits with "IVCT" -- Part II. Application of "IVCT" to various mouse organs -- 5. Histochemical Analyses of Living Mouse Liver under Different Hemodynamic Conditions -- 6. Three-dimensional reconstruction of liver tissues with confocal laser scanning microscopy -- 7. Application of "in vivo cryotechnique" to immunohistochemical detection of hypoxia in mouse liver tissues treated with pimonidazole -- 8. Immunohistochemical detection of soluble immunoglobulins in small intestines -- 9. Detection of MAPK signal transduction proteins in an ischemia/reperfusion model of small intestines -- 10. Histological Study and LYVE-1 Immunolocalization of Mesenteric Lymph Nodes -- 11. Distribution of immunoglobulin-producing cells in immunized mouse spleens -- Chapter12. Alteration of erythrocyte shapes in various organs of living mice or under blood flow conditions -- 13. Dynamic ultrastructure of smooth muscle cells in dystrophin-deficient mdx or normal scn mice -- 14. Dynamic Bioimaging of Serum Proteins in Beating Mouse Hearts -- 15. Histochemical analyses and quantum dot imaging of microvascular blood flow with pulmonary edema -- 16. Dynamic ultrastructure of pulmonary alveoli of living mice under respiratory conditions -- 17. Immunohistochemical analysis of various serum proteins in living mouse thymus -- 18. Immuno- and enzyme-histochemistry of HRP for demonstration of blood vessel permeability in mouse thymic tissues -- 19. Immunolocalization of serum proteins in living mouse glomeruli under various hemodynamic conditions -- 20. Immunohistochemical Analyses on Albumin and IgG in Acute Hypertensive Mouse Kidneys -- 21. Application of novel "in vivo cryotechnique" in living animal kidneys -- 22. Application of periodic acid-Schiff fluorescence emission for imunohistochemistry of living mouse renal glomeruli -- 23. Immunohistochemical analyses on serum proteins in nephrons of protein-overload mice -- 24. Immunohistochemical analyses of serum proteins in puromycin aminonucleoside nephropathy of living rat kidneys -- 25. Immunolocalization of phospho-Arg-directed protein kinase-substrate in hypoxic kidneys -- 26. Dynamic ultrastructures of renal glomeruli in living mice under various hemodynamic conditions -- 27. Function of dynamin-2 in formation of discoid vesicles in urinary bladder umbrella cells -- 28. Involvement of follicular basement membrane and vascular endothelium in blood-follicle barrier formation of mice -- 29. Permselectivity of blood-follicle barriers in mouse polycystic ovary model -- 30. Application of "in vivo cryotechnique" to immunohistochemical study of serum albumin in normal and cadmium-treated mouse testis organs -- 31. Immunohistochemical detection of angiotensin II receptors in mouse cerebellum -- 32. Application of "in vivo cryotechnique" to immunohistochemical analyses for effects of anoxia on serum immunoglobulin and albumin leakage through blood-brain barrier in mouse cerebellum -- 33. Extracellular Space in Central Nervous System -- 34. Overview on recent applications of in vivo cryotechnique in neurosciences -- 35. Application of "in vivo cryotechnique" to immunohistochemical detection of phosphorylated rhodopsin in light-exposed retina of living mouse -- 36. Application of "in vivo cryotechnique" to immunohistochemical detection of glutamate in mouse retina inner segment of photoreceptors -- 37. Application of ǣin vivo cryotechniqueǥ to immunohistochemical study of mouse sciatic nerves under various stretching conditions -- Part III. Further preparation after "IVCT" -- 38. Freeze-substitution (FS) fixation method -- Part IV. Comments on advantages of "IVCT" -- 39. Cryotechniques and freeze-substitution for immunostaining of intranuclear antigens and fluorescence in situ hybridization -- 40. Replica immunoelectron microscopy for caveolin in living smooth muscle cells -- 41. Application of "in vivo cryotechnique" to detection of injected fluorescence-conjugated IgG in living mouse organs -- 42. Application of "in vivo cryotechnique" to visualization of microvascular blood flow in mouse kidney by quantum dot injection -- Part V. Direct detection of molecules by Raman microscopy -- 43. Application of "in vivo cryotechnique" to Raman microscopy of freeze-dried mouse eyeball-slice -- 44. Application of "in vivo cryotechnique" to detection of erythrocyte oxygen saturation in mouse blood vessels with confocal Raman cryomicroscopy -- Part VI. Future development for human pathology -- 45. Imaging of thrombosis and microcirculation in mouse lungs of initial melanoma metastasis -- 46. Differential distribution of blood-derived proteins in xenografted human adenocarcinoma tissues -- Part VII. New cryobiopsy invention -- 47. Morphological and Histochemical Analysis of Living Mouse Livers by New "Cryobiopsy" Technique -- 48. Recent Development of In Vivo Cryotechnique to Cryobiopsy for Living Animals -- Part VIII. Application of cryobiopsy for living animal tissues -- 49. Significance of cryobiopsy for morphological and immunohistochemical analyses of xenografted human lung cancer tissues and functional blood vessels -- 50. Immunolocalization of serum proteins in xenografted mouse model of human tumor cells -- 51. Histochemical approach of cryobiopsy for glycogen distribution in living mouse livers under fasting and local circulation loss conditions -- Part IX. New bioimaging of living animal tissues -- 52. Bioimaging of fluorescence-labeled mitochondria in subcutaneously grafted murine melanoma cells by the 'in vivo cryotechnique" -- 53. Application of "in vivo cryotechnique" to visualization of ATP with luciferin-luciferase reaction in mouse skeletal muscles -- 54. Concluding remarks.
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e-Book
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Reflectance Confocal Microscopy for Skin Diseases

https://libcat.nshealth.ca/en/permalink/provcat31129
Rainer Hofmann-Wellenhof ... [et al.], editors. --Berlin, Heidelberg: Springer , c2012.
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In recent years the relevance of non-invasive bioimaging techniques in the field of melanoma screening has steadily increased. In the new era of "clinicoimaging" diagnosis, reflectance confocal microscopy (RCM) will have a major impact on the diagnosis and management of neoplastic and inflammatory skin diseases. This book focuses on the use and significance of in vivo RCM for non-invasive high-resolution imaging of the skin. All of the chapters in this hands-on guide are generously illustrated…
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Other Authors
Hofmann-Wellenhof, Rainer
Responsibility
Rainer Hofmann-Wellenhof ... [et al.], editors
Place of Publication
Berlin, Heidelberg
Publisher
Springer
Date of Publication
c2012
Physical Description
1 online resource (ix, 500 p. : 507 ill., 485 ill. in color)
ISBN
9783642219979
Subjects (MeSH)
Microscopy, Confocal
Skin Diseases - diagnosis
Skin Diseases - pathology
Skin Neoplasms - diagnosis
Skin Neoplasms - pathology
Subjects (LCSH)
Dermatology
Abstract
In recent years the relevance of non-invasive bioimaging techniques in the field of melanoma screening has steadily increased. In the new era of "clinicoimaging" diagnosis, reflectance confocal microscopy (RCM) will have a major impact on the diagnosis and management of neoplastic and inflammatory skin diseases. This book focuses on the use and significance of in vivo RCM for non-invasive high-resolution imaging of the skin. All of the chapters in this hands-on guide are generously illustrated with numerous confocal images and structured in a reader-friendly way. The contents include: a detailed information on the most relevant and up-to-date aspects of RCM, schematic drawings summarizing and explaining the most important RCM criteria, and a chapter specifically devoted to bridging the gap between dermoscopy, RCM, and histopathology. At the end of each chapter, core messages recapitulate the most pertinent aspects. Reflectance Confocal Microscopy for Skin Diseases will be a valuable resource for all physicians involved in the diagnosis and treatment of neoplastic and inflammatory skin diseases.
Contents
[Machine generated contents note] Part I. Confocal Reflectance Microscopy: The Essentials -- 1. Confocal Story / Hans Peter Soyer -- 2. How Reflectance Confocal Microscopy Works / Ralph P. Braun -- 3. Hands-on Guide to Confocal Imaging / Verena Ahlgrimm-Siess -- Part II. Normal Skin -- 4. Epidermis, Dermis and Epidermal Appendages / Joanne Rocha-Portela -- 5. Acral Volar Skin, Facial Skin and Mucous Membrane / Myrna Hanke-Martinez -- Part III. Melanocytic Lesions -- 6. Semeiology and Pattern Analysis in Melanocytic Lesions / Giovanni Pellacani -- 7. Dermoscopic and Histopathologic Correlations / Giovanni Pellacani -- Part IV. Melanocytic Lesions: Nevi -- 8. Common Nevi / Giovanni Pellacani -- 9. Atypical/Dysplastic Nevi / Melissa Gill -- 10. Spitz Nevi / Giovanni Pellacani -- 11. In Vivo Confocal Reflectance Microscopy of Congenital Melanocytic Nevi / Alon Scope -- 12. Many Faces of Nevi: Blue, "Black" and Recurrent Nevi / Alon Scope -- Part V. Melanocytic Lesions: Melanoma -- 13. Superficial Spreading Melanoma / Giovanni Pellacani -- 14. Melanoma Progression / Giovanni Pellacani -- 15. Nodular Melanoma / Sonia Segura -- 16. Lentigo Maligna / Pascale Guitera -- 17. Amelanotic Melanoma / Pascale Guitera -- Part VI. Nonmelanocytic Skin Lesions -- 18. Semiology and Pattern Analysis in Nonmelanocytic Lesions / Susana Puig -- 19. Dermoscopic and Histopathologic Correlations / Susana Puig -- 20. Solar Lentigo, Seborrheic Keratosis and Lichen Planus-Like Keratosis / Rainer Hofmann-Wellenhof -- 21. Basal Cell Carcinoma / Alon Scope -- 22. Actinic Keratosis / Susanne Astner -- 23. Squamous Cell Carcinoma / Harold S. Rabinovitz -- 24. Cutaneous Lymphoma / Rainer Hofmann-Wellenhof -- 25. Potpourri of Nonmelanocytic Skin Lesions / Giuseppe Argenziano -- Part VII. Inflammatory Skin Diseases -- 26. Semiology and Patterns of Inflammatory Skin Conditions / Marina Agozzino -- 27. Hyperkeratotic Dermatitis / Marco Ardigo -- 28. Spongiotic Dermatitis / Martina Ulrich -- 29. Interface Dermatitis / Marco Ardigo -- 30. Pigmentary Skin Disorders / Marco Ardigo -- Part VIII. Monitoring of Skin Lesions and Therapy Control -- 31. Follow-up of Nevi / Verena Ahlgrimm-Siess -- 32. Monitoring of Nonsurgical Treatment of Skin Tumors / Verena Ahlgrimm-Siess -- 33. Confocal Mosaicing Microscopy in Skin Excisions: Feasibility of Cancer Margin Screening at the Bedside to Guide Mohs Surgery / Milind Rajadhyaksha -- 34. Reflectance Confocal Microscopy Applications in Cosmetology / Marco Ardigo -- Part IX. Future Aspects -- 35. Tele-Reflectance Confocal Microscopy / Giovanni Pellacani -- 36. Automated Diagnosis and Reflectance Confocal Microscopy / Marco Wiltgen -- 37. Experimental Applications and Future Directions / James M. Zavislan -- Part X. Glossary -- 38. Reflectance Confocal Microscopy Imaging: A Glossary of Terminology / Alon Scope.
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The slit lamp : applications for biomicroscopy and videography

https://libcat.nshealth.ca/en/permalink/provcat33786
Marcus-Matthias Gellrich ; with a contribution by Dieter Schmidt ; English translation by Carole Gustely Cürten. --Heidelberg: Springer , c2014.
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In this book the author describes in detail the history, construction, and examination potential of the slit lamp. In particular, however, he presents a new approach videography that allows the ophthalmologist to document all (!) eye diseases with the slit lamp and a video camera in a practicable, rapid, and affordable manner. The necessary methods, techniques, and equipment, including converging, diverging, and contact lenses, are clearly explained in the text and four didactic videos. The 20 …
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Author
Gellrich, Marcus-Matthias
Responsibility
Marcus-Matthias Gellrich ; with a contribution by Dieter Schmidt ; English translation by Carole Gustely Cürten
Place of Publication
Heidelberg
Publisher
Springer
Date of Publication
c2014
Physical Description
1 online resource (xvii, 218 pages)
ISBN
9783642397936 (electronic bk.)
9783642397929
Subjects (MeSH)
Diagnostic Techniques, Ophthalmological - instrumentation
Eye Diseases - diagnosis
Microscopy, Video
Subjects (LCSH)
Ocular biomicroscopy
Ophthalmology
Médecine
Abstract
In this book the author describes in detail the history, construction, and examination potential of the slit lamp. In particular, however, he presents a new approach videography that allows the ophthalmologist to document all (!) eye diseases with the slit lamp and a video camera in a practicable, rapid, and affordable manner. The necessary methods, techniques, and equipment, including converging, diverging, and contact lenses, are clearly explained in the text and four didactic videos. The 20 most important videographic settings are described, and recipes for their use are presented, along with diagnostic tips. The accompanying collection of clinical images represents the world's first ever general atlas of ophthalmology from the perspective of the slit lamp. The author has already demonstrated proof of the value of ophthalmological videography in his own practice. It is his hope that this introductory book, with its many images never previously produced with a slit lamp, will stimulate others to exploit the approach's potential.
Contents
1. Basics -- 2. Biomicroscopy -- 3. Videography: Basics -- 4. Special Videography -- 5. Videographic Settings for Specific Diseases -- 6. History of the Slit Lamp.
Format
e-Book
Location
Online
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Technology in Practical Dermatology : Non-Invasive Imaging, Lasers and Ulcer Management

https://libcat.nshealth.ca/en/permalink/provcat45292
edited by Michele Fimiani, Pietro Rubegni, Elisa Cinotti. --Cham: Springer , c2020.
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This book provides a complete overview on the latest available technologies in dermatology, while discussing future trends of this ever-growing field. This handy guide provides clinicians and researchers with a clear understanding of the advantages and challenges of laser and imaging technologies in skin medicine today. It also includes a section on imaging techniques for the evaluation of skin tumors, with chapters devoted to dermoscopy, in vivo and ex vivo reflectance confocal microscopy, hig…
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Other Authors
Fimiani, Michele
Rubegni, Pietro
Cinotti, Elisa
Responsibility
edited by Michele Fimiani, Pietro Rubegni, Elisa Cinotti.
Place of Publication
Cham
Publisher
Springer
Date of Publication
c2020
Physical Description
1 online resource (xviii, 500 p.) : 242 illus., 233 illus. in color
ISBN
9783030453510
9783030453503 (Print ed.)
9783030453527 (Print ed.)
9783030453534 (Print ed.)
Subjects (MeSH)
Laser Therapy - methods
Microscopy - methods
Skin Diseases - diagnostic imaging
Wounds and Injuries - therapy
Specialty
Dermatology
Diagnostic Imaging
Laser Therapy
Abstract
This book provides a complete overview on the latest available technologies in dermatology, while discussing future trends of this ever-growing field. This handy guide provides clinicians and researchers with a clear understanding of the advantages and challenges of laser and imaging technologies in skin medicine today. It also includes a section on imaging techniques for the evaluation of skin tumors, with chapters devoted to dermoscopy, in vivo and ex vivo reflectance confocal microscopy, high frequency ultrasound, optical coherence tomography, and a closing part on latest approaches to wound management. Completed by over 200 clinical images, Current Technology in Practical Dermatology: Non-Invasive Imaging, Lasers and Ulcer Management is both a valuable tool for the inpatient dermatologist and for physicians, residents, and medical students in the field.
Contents
Part I. Imaging techniques for the evaluation of skin diseases -- 1. Dermoscopy: fundamentals and technology advances -- 2. Dermoscopy for benign melanocytic skin tumors -- 3. Dermoscopy for melanoma -- 4. Dermoscopy for non-melanocytic benign skin tumors -- 5. Demoscopy for non-melanocytic malignant skin tumors -- 6. Dermoscopy for inflammatory diseases -- 7. Dermoscopy for infectious diseases -- 8. Digital dermoscopy analysis -- 9. Optical super-high magnification dermoscopy -- 10. Fluorescence videodermoscopy -- 11.Total body photography and sequential digital dermoscopy for melanoma diagnosis -- 12. History and fundamentals of reflectance confocal microscopy -- 13. In vivo reflectance confocal microscopy for benign melanocytic skin tumors -- 14. In vivo reflectance confocal microscopy for melanoma -- 15. In vivo reflectance confocal microscopy for non melanocytic benign skin tumors -- 16. In vivo reflectance confocal microscopy for non melanocytic malignant skin tumours -- 17. In vivo reflectance confocale microscopy for inflammatory diseases -- 18. In vivo reflectance confocal microscopy for infectious diseases -- 19. In vivo reflectance confocal microscopy for mucous membranes -- 20. Ex vivo confocal microscopy -- 21. Ultrasound -- 22. Optical coherence tomography -- 23. High-definition optical coherence tomography -- 24. 3D imaging -- 25. Raman spectroscopy -- 26. Multispectral and hyperspectral imaging for skin acquisition and analysis -- 27. Electrical impedance in dermatology -- Part II. Lasers and light sources technologies in dermatology -- 28. Laser light and light-tissue interaction -- 29. Laser and light sources: safety and organization issues -- 30. Intense polichromatic lights and light emitting diodes: what's new -- 31. Vascular lasers: tips and protocols -- 32. Broadband intense pulsed lights for vascular malformations -- 33. Pigment specific lasers for benign skin lesions and tattoos: long pulsed, nanosecond and picosecond lasers -- 34. Skin resurfacing: ablative and non-ablative lasers -- 35. Photorejuvenation: concepts, practice, perspectives -- 36. Laser hair removal: updates -- 37. Biophotonic therapy induced photobiomodulation -- 38. Photodynamic therapy -- Part III. Technological advances in wound management -- 39. Temporary dressing -- 40. Extracellular matrices -- 41. Skin bank bioproducts: the basics -- 42. Clinical applications of skin bank bioproducts -- 43. Negative pressure wound therapy -- 44. Tissue-engineered skin substitutes -- 45. Biologics in wound management -- 46. Stem cell in wound healing -- Part IV. New complementary tools for dermatologic diagnosis -- 47. Microbiopsy in dermatology -- 48. Noninvasive genetic testing: adhesive patch-based skin biopsy and buccal swab -- 49. Liquid biospsies.
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e-Book
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Online
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